%一个姚明,Guangzhe %苗,鑫鑫%吴,Mengxuan % Lv, Zhenguo %呗,Yu % Chang, Yanxu %欧阳,惠子%他,小君% D J前沿2023%药理学% C % F % G英语% K榄仁树属chebula R雷竞技rebatetz。提取物、活性化合物,药代动力学,UPLC-MS /女士,大鼠血浆% Q % 10.3389 R / W fphar.2023.1067089 % % L % M % P % 7% 8 2023 - 1月- 13 % 9原始研究% # % !药代动力学的榄仁树属chebula Retz。% * % < % T的药物活性化合物的榄仁树属chebula Retz。Ethanolic提取后大鼠口服使用UPLC-MS / % U //www.thespel.com/articles/10.3389/fpha雷竞技rebatr.2023.1067089 % V女士14% 0期刊文章% @ 1663 - 9812 % X榄仁树属chebula Retz。(TC)是一个著名的中药和丰富的化学成分具有多种药理作用。在这项研究中,超高效液体chromatography-tandem质谱仪(UPLC-MS / MS)方法开发和用来确定9活性化合物的血药浓度(chebulic酸、没食子酸、原儿茶酸、corilagin, chebulagic酸,chebulinic酸,1,2,3,4,6-O-pentagalloylglucose,鞣花酸和没食子酸乙酯)口服后TC在老鼠身上提取。血浆样品的预处理与甲醇蛋白沉淀,和咖啡酸被用来作为内部标准(是)。化合物精度内部和inter-day不到14.6%,和准确性从−11.7%到13.5%不等。化合物的提取回收率在84.9%和108.4%之间,而基体效应发生在86.4%和115.9%之间。 Stability tests showed that all nine analytes had been stable under four storage conditions, and statistically significant the relative standard deviations were under 13.7%. The validated UPLC-MS/MS method was applied with great success to plasma pharmacokinetics analysis of the TC extracts, and the pharmacokinetic results showed that among the nine components, the area under the concentration-time curve (AUC_(0万亿),231112.38±64555.20 h ng / mL)和最大浓度(C_马克斯,4983点±1721.53 ng / mL) chebulagic酸相对较大,这表明它有一个更高层次的等离子体曝光。消除半衰期(T_1/2)chebulinic酸,corilagin chebulagic酸分别为43.30,26.39和19.98 h,分别,这表明这些分析物表明长期保留和体内代谢更慢。本研究将提供一个理论基础为进一步TC在临床实践中的应用。