< p > <大胆>简介:< /大胆>天然来源的生物活性分子在为现代激素类避孕药避孕性能优良的替代品。世界各地的研究人员正在确定避孕导致针对男性生殖系统而不是通常的女性避孕药。缺乏正确理解男性避孕药物靶点蛋白导致证据不足的活动确定了避孕的化合物。蛋白质特定于男性生殖系统和参与精卵融合目标识别将是一个很好的药物男性无激素,可逆避孕线索。抑制精子透明质酸酶活性的天然无激素化合物会导致可逆和无激素的男性避孕。的Aegle marmelos绝壁。就是这样一种重要的药用植物有价值的phytocompounds,传统上作为一个潜在的避孕措施。叶提取物的体内实验Aegle marmelos。林含萜烯、甾醇类和生物碱显示突出避孕的活动。此外,本研究探讨了潜在能力的叶提取物抑制精子透明质酸酶行动与其他分子之间的相互作用的细节精子透明质酸酶和三个phytocompounds aegeline等marmin, marminol。< / p > < p > <大胆>材料和方法:< /大胆>透明质酸酶体外抑制试验和计算机辅助精子分析(CASA)是用来评估的男性避孕属性Aegle marmelos绝壁。 leaf extract. To identify the interaction profile of aegeline, marmin, and marmenol on sperm cell hyaluronidases the in-silico methods such as molecular docking, Non-Covalent Interaction analysis, Molecular dynamics, and Molecular Mechanics Poisson Boltzmann Surface Area were used.Results and discussion: The results of in vitro hyaluronidase inhibition assay and Computer Assisted Sperm Analysis shows the inhibition of hyaluronidase enzymatic activity and reduced sperm activities in the presence of leaf extracts. After incubation with leaf extracts for about 30 minutes time intervals show, the motility drops from progressive to non-progressive and ended up with complete immotile in 100 μg/ml concentration of leaf extract. The results of molecular docking, Non-Covalent Interaction analysis, Molecular dynamics, and Molecular mechanics Poisson Boltzmann Surface Area show that the phytocompounds marmin, and aegeline have the potential ability to inhibit sperm hyaluronidase.